Alonso-Stepanova Lab@AlonsoStepanova

Plant Synthetic Biology. Translation Regulation. Plant Hormones. Genome Editing. Recombineering. DIY Biology

 NC State University
alonsostepanova.wordpress.nc…
Joined July 2018
19 Photos and videos
Alonso-Stepanova Lab retweeted
Mario Fenech@Mr_FenTor
Aug 17
🌿🪴NEW PREPRINT ALERT🪴🌿 @AlonsoStepanova 1/5 🌱How do plants coordinate hormone signals to decide when and how much to grow? 🧵👇 biorxiv.org/content/10.64898…

ALT Breaking Despicable Me GIF by Regal

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Excited to share Mario’s latest manuscript, now up on bioRxiv! Using new single-locus multi-hormone reporters, Mario and the team dissected the spatially resolved Auxin-Cytokinin-Ethylene crosstalk hierarchy driving root growth inhibition in Arabidopsis. biorxiv.org/content/10.64898…

An Integrated Spatially Resolved Mechanistic Model of Hierarchical Auxin–Cytokinin–Ethylene...

Decoding how plants integrate multiple hormone signals to coordinate growth requires tools capable of resolving pathway interactions at cellular resolution in living tissue. Here we present ACE...

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We found distinct spatial roles for different hormones: Ethylene boosts auxin biosynthesis (TAA1) & imports it (AUX1) into outer root cells to block cell elongation. Cytokinin keeps ethylene-boosted auxin in the inner vasculature & lateral root cap to shrink the meristem (RAM).
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Bottom line: Ethylene acts as the central hub for auxin activation, while cytokinin acts as a spatial manager directing where that auxin goes. ACE2 overcomes early technical bottlenecks, opening the door for higher-order hormone monitoring across plant bio! 🌱✨
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The Alonso-Stepanova lab at NCSU is hiring a postdoc to build a programmable, reversible transcriptional switch (SCRIBE) to control gene expression in plants 🌱 🔬 Project: nsf.gov/awardsearch/show-awa… 💼 Apply: jobs.ncsu.edu/postings/23284… Please RT & share!
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@AlonsoStepanova It has been a great start to the summer in the Alonso-Stepanova lab. First, Jose was elected to the National Academy of Sciences, and just yesterday, Anna was appointed as a William Neal Reynolds Distinguished Professor. Two great honors for our lab.
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Alonso-Stepanova Lab retweeted
Roeder lab@RoederLab
Feb 14
A Postdoctoral Associate position is available in the Roeder Laboratory at Cornell University in Ithaca, NY with a focus on researching Polyploidy. Apply by March 1. Please spread the word. apps.hr.cornell.edu/recruiti…
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Congrats to Anna Yaschenko on the 6th paper from her dissertation work that is out today. It's a review on plant synthetic promoters that stems from the intro chapter of her PhD thesis. One more to go! frontiersin.org/journals/pla…

Frontiers | Synthetic promoter design in plants: integration of computational and experimental...

Understanding how to engineer transcriptional regulation in plants is key to advancing both fundamental knowledge and practical applications in plant biology...

frontiersin.org
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Alonso-Stepanova Lab retweeted
Catharina Merchante / Karen@k_merchante
Jan 4
It was great to collaborate with you @AlonsoStepanova, as always 🤩 Congrats, Jade, for an amazing job! 🌱🧬🩷
The Plant Journal@ThePlantJournal
Jan 4
📚𝐅𝐨𝐮𝐧𝐝𝐚𝐭𝐢𝐨𝐧 𝐑𝐞𝐯𝐢𝐞𝐰: Translational control in plants: from basic mechanisms to environmental and developmental responses 🧬🌱 ✍️ Jade Lyons, @k_merchante, @AlonsoStepanova Discover the power of translation in shaping plant responses👉 doi.org/10.1111/tpj.70647
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Out now in @ThePlantJournal: A deep dive into translation in plants by Jade & Karen! 🧬🌱 What started as Jade’s prelim evolved into a fantastic review on plant translation, thanks to a great collaboration with Karen. Congrats on this excellent review! onlinelibrary.wiley.com/doi/…

Translational control in plants: from basic mechanisms to environmental and developmental responses

Translation is a key regulatory point in the control of gene expression. This review provides an overview of the translation process and molecular machinery that underlie it, highlights the major...

onlinelibrary.wiley.com
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We attempted to "clean up" the model of the auxin biosynthesis pathway in Arabidopsis using genetic, pharmacological, and metabolomic approaches. We show that the CYP71A, NIT, AMI, and IAMH gene families are all dispensable for auxin production via IAOx. academic.oup.com/plcell/arti…

The CYP71A, NIT, AMI, and IAMH gene families are dispensable for indole-3-acetaldoxime-mediated...

Research challenges previously postulated genetic pathways for the conversion of indole-3-acetaldoxime into indole-3-acetic acid in the auxin-overproducing

academic.oup.com
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It was a busy summer at the Alonso-Stepanova Lab with four manuscripts now published. Pathy, Mario, and Anna's EBSn ethylene reporter paper is now available in the Plant Biotechnology Journal. onlinelibrary.wiley.com/doi/…

EBSn, a Robust Synthetic Reporter for Monitoring Ethylene Responses in Plants

Ethylene is a gaseous plant hormone that controls a wide array of physiologically relevant processes, including plant responses to biotic and abiotic stress, and induces ripening in climacteric...

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Chengsong's DASH cloning system for high-cargo gene stacking has also been published in the Plant Biotechnology Journal. onlinelibrary.wiley.com/doi/…

DASH: a versatile and high‐capacity gene stacking system for plant synthetic biology

DNA assembly systems based on the Golden Gate method are popular in synthetic biology but have several limitations: small insert size, incompatibility with other cloning platforms, DNA domestication...

onlinelibrary.wiley.com
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And last but not least, Chengsong and Katie's wonderful collaboration with Chase Beisel's group on a new genome editing tool based on targeted DNA ADP-ribosylation, is now published in Nature Biotechnology. nature.com/articles/s41587-0…

Targeted DNA ADP-ribosylation triggers templated repair in bacteria and base mutagenesis in...

Nature Biotechnology - Append editing of ADP-ribosyl to thymine is used for precise modifications in bacteria and eukaryotes.

nature.com
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The Alonso-Stepanova lab at NC State is hiring a postdoc! Join us to study plant regeneration in Arabidopsis using cutting-edge molecular genetics and synthetic biology. We're looking for a driven and ambitious recent PhD graduate. Apply here: jobs.ncsu.edu/postings/22206…
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In the latest Mario's preprint, we show that the CYP71A, NIT, AMI, and IAMH gene families do not play a role in the IAOx-mediated auxin biosynthesis biorxiv.org/content/10.1101/…

The CYP71A, NIT, AMI, and IAMH gene families are dispensable for indole-3-acetaldoxime-mediated...

Indole-3-acetic acid (IAA) is a crucial auxin governing plant development and environmental responses. While the indole-3-pyruvic acid (IPyA) pathway is the predominant IAA biosynthesis route, other...

biorxiv.org
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Our genetic and metabolic analysis shows that knocking out the CYP71A, NIT, and AMI gene families doesn't lead to auxin-deficient phenotypes or suppress the high-auxin defects of the sur2 mutant. This questions the physiological role of these genes in auxin production.
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Our work points to an undiscovered auxin biosynthesis pathway involving IAOx, particularly active in sur2 mutants. 🔎 Uncovering the genes and metabolic steps of this new route could improve our understanding of IAA synthesis in plants!
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