ALT A diagram outlining the difference between PCR and CRISPR based pipelines for DNA barcoding. Unlike PCR-based approaches where PCR reagents must successfully amplify target regions and sequenced on a lab-based sequencer, CRISPR based approaches enrich target regions using the CRISPR-Cas complex and sequenced using portable sequencing technologies (e.g., ONT). Where PCR-based approaches may be laborious and require expensive equipment, CRISPR-based methods are faster, more versatile and precise.