And it's finally out! DNA-PAINT tracking goes live cell and dual colour! Kudos to @NiederauerC and Chi Nguyen from our lab and thanks the Jungmann and Schwille labs @MPI_Biochem for the great collaboration 🎉

So, if you want to build a stable, multicolor single-molecule TIRF microscope 🔬, or upgrade yours with a few home-built bits and bobs, look no further (kudos to @NiederauerC 👏) 👇

If you're curious about how we use DNA-PAINT-SPT to study protein-protein interaction on membranes, come visit poster 59 at #SMLMS this evening

Do you have experience in (single-molecule) imaging or protein reconstitution from your PhD? Do you like the idea of a collaborative project focused on immunotherapy? Then check out our postdoc vacancy 👇 - drop me an email if interested. Please retweet! amolf.nl/jobs/postdoc-discov…

...and it's finally out! If you are doing SPT, check out how to greatly improve trajectory lengths by DNA-PAINT tracking! Great collaboration & great work by @_SteinJ , Florian Sehr, @NiederauerC and @julian_bauer 🎉

How to track single particles for up to tens of minutes via DNA-mediated fluorophore exchange? Read about the latest collaboration between the Schwille, @KGanzinger, and Jungmann labs out now in @NatureComms: ➡️nature.com/articles/s41467-0… #SMLM #DNA-PAINT #SingleParticleTracking

#ebsa_in attendees: I'll present the technique and it's application for single-molecule tracking of membrane proteins on Sunday afternoon at the advanced optical microscopy session

Thrilled and honoured to receive a 800.000€ @nwo #vidi grant to research immune cell signalling using synthetic biology and single-molecule tools 🥳 Very thankful to my group & colleagues at @AMOLF & elsewhere for invaluable feedback - I look forward to kicking off this project!

Finally uploaded the plans and component list of our single-molecule TIRF microscope: ganzingerlab.github.io/K2TIR… #miCube

Tracking Single Particles for Hours via Continuous DNA-mediated Fluorophore Exchange biorxiv.org/cgi/content/shor… #biorxiv_biophys

Focus lock / z-drift correction on our TIRF setup: back reflection of a 808nm laser in TIR is recorded by a CCD array and shifts when the sample is translated in z. #microscopemonday #micube check out big.umassmed.edu/wiki/index.… #kbellve

Oheim et al. review methods for calibrating the TIRF evanescent field and push for making quantitative TIRF data more comparable!

Check out our latest work on facilitating the calibration of TIRF setups, published in Optics Express.

Read the paper "Direct characterization of the evanescent field in objective-type total internal reflection fluorescence microscopy" of the Schwille Lab. osapublishing.org/oe/abstrac…