Making every day in lab as unique as every single cell | @shaleklab | Harvard-MIT @mit_hst | @HertzFoundation Fellow | @NSFGRFP Fellow
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What can we learn from all cytokines in the human genome? For most, we have limited info on what they do and when to target them
Meet TAPAS & EPICURE: tools to synthesize all human cytokines➡️scRNA data of primary human immune responses➡️🖥️methods to use them in your work (1/n)
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Here’s another demo, for progranulin: normally made as a single large protein, but can be proteolytically cleaved into individual domains. We synthesized them all with TAPAS and showed one polypeptide can drive many shared vs. divergent responses in downstream cells (6/n)
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And many thanks for support from @BiswasFamilyFdn, @10xGenomics @ChanZuckerberg via 100M Cells Challenge, @broadinstitute BroadIgnite, and many more collaborators @kochinstitute @MIT @broadinstitute @MIT_IMES @ragoninstitute @mit_hst @harvardmed @ChemistryMIT @wyssinstitute(13/n)
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That’s all for now, folks – see here for a link to preprint (fin/n) biorxiv.org/content/10.64898…
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What can we learn from all cytokines in the human genome? For most, we have limited info on what they do and when to target them
Meet TAPAS & EPICURE: tools to synthesize all human cytokines➡️scRNA data of primary human immune responses➡️🖥️methods to use them in your work (1/n)
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For broad disease relevance, we made all cytokines in the human genome➡️stimulate primary human PBMCs➡️scRNA perturbation datasets. Proteins group together around the shared downstream responses they drive in primary human immune cells (4/n)
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We created TAPAS to synthesize genome-scale protein signals, then measure how they regulate primary human cells’ healthy functions and disease responses. Human-based cell-free protein synthesis lets us do this in high-throughput, even by a single scientist in just 48 hours! (3/n)
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90% of data focuses on 10% of cytokines. Which cytokines are most studied today is best predicted by their historical discovery order, back to the 1970s. Millions of patients are on therapies targeting already-well-studied signals – what can the rest of signals do? (2/n)
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35th Annual Arias Symposium coming up soon on 12/2! Register for some fantastic speakers and liver community :) alfevents.org/ariassymposium…
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Apply to 2026 yeast display course from @ipiproteins! I did last year's - incredibly knowledgeable and helpful instructors, lovely community, fantastic one-week speedrun to start using yeast display yourself. A very enriching experience 🥁✨
Due Mar 30 proteininnovation.org/yeast-…
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Constantine Tzouanas retweeted
The new issue is out! 👉cell.com/cell/current
On the cover, Tzouanas et al. show how environmental stressors like high-fat diets challenge the social contract unifying cellular citizens, pushing some cells to prioritize their own survival over collective community goals.
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Incredible to see our work on @CellCellPress cover! Environmental stresses like high-fat diets➡️cellular communities whose past histories & present neighbors shape future disease progression & cancer outcomes (with Akira-inspired Easter eggs too!). Appreciation to @TeamSciStories
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Link to X-planation summary of the paper
Out today in @CellCellPress! We studied how environmental stresses like high-fat diets today can prime tumorigenesis months to years in the future (1/n)
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And link to the paper itself cell.com/cell/fulltext/S0092…
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