@HopkinsMedicine & @IITKgp alum | Former K99 & @DamonRunyon fellow at @UCSanDiego
San Diego, CA
- Tweets 1,057
- Following 514
- Followers 556
- Likes 3,904
Pinned Tweet
Our work on the architecture and model of the #NuclearBasket is now out @CellCellPress. cell.com/cell/pdf/S0092-8674…. @HHMINews, @IntActOme #NPC #teamtomo
A short tweetorial follows
7
19
110
22,232
Digvijay Singh retweeted
Nuclear Pore Complexes (NPCs) are the gateways to the nucleus, but how can they be both rapid and picky, even for very large cargoes? To answer this question, we built the most detailed data-driven model to date of nucleocytoplasmic transport:
pnas.org/doi/10.1073/pnas.25…
1
5
18
3,550
Digvijay Singh retweeted
Doing a very rough analysis (very dirty data) of biopharmas with over $100m raised. This is what i’m getting and it seems very wrong. But maybe its my intuition that is off (?).
Boston Metro: 183 companies
Bay Area Metro: 102 companies
San Diego Metro: 187 companies
4
1
22
3,847
Check out this cool work!
Kudos to @ReikaWatanabe2 for leading the establishment of the #InSitu #cryoET pipeline at #LJI. She is such a powerhouse!
Our in-situ structural analysis of the Ebola virus nucleocapsid is now published in @CellCellPress! Using cryo-FIB-ET, we revealed a 9Å structure, identifying novel layers and conserved interfaces that may serve as antiviral targets. #Ebola #Virology #StructuralBiology
1
5
922
.@LuskingL have written a wonderful preview on our basket work. Thanks!
cell.com/cell/abstract/S0092…
1
5
23
1,613
Digvijay Singh retweeted
From preprint to journal. Our nuclear gatekeepers have successfully unlocked the gates to Cell (Journal) cell.com/action/showPdf?pii=… #NPC #integrativemodeling Congrats to team @dgvjayS @JoshHutchings94 @ig_ech @RoutLab_RU @salilab_ucsf @TheVillaLab Animation piped.video/watch?v=LiKdFIxr…
Tiny gatekeepers of our nucleus decide what gets in and out. Follow our study on Yeast and Mammalian NPC baskets and unravel the molecular structure of these cellular bouncers! Don't miss the side-by-side comparison in the video. #IntegrativeModeling biorxiv.org/content/10.1101/…
4
18
60
5,648
Our work on the architecture and model of the #NuclearBasket is now out @CellCellPress. cell.com/cell/pdf/S0092-8674…. @HHMINews, @IntActOme #NPC #teamtomo
A short tweetorial follows
7
19
110
22,232
Leveraging in-cell #cryoEM, we explored the neighborhood of different NPCs and found observed a clear chromatin exclusion around the mammalian NPC & created a 3D map of the basket with this surrounding exclusion, highlighting its potential impact on #ChromatinOrganization.
1
6
606
Shout out to all the wonderful authors & institutions!
@neeleshsoni03 @JoshHutchings94 @ig_ech @M_C_Duquette @_themovingvan_ @TheVillaLab @salilab_ucsf @RoutLab_RU Javier @QiangGuo_EM Brian @Proteomics_Shi & others
@UCSanDiego @HHMINews @IntActOme @UCSF @DamonRunyon @NIHAging
1
3
1,366
Excited to have this #teamtomo book finally out!
Here's our chapter on #CryoFIBMilling, where we distilled the wisdom of >170 manuscripts. link.springer.com/chapter/10…
Thanks to @BriegelAriane & @FriedrichForst1 for editing this giant book!
Finally- a book on cryo-electron tomography, written by leading experts! Edited by the one and only @FriedrichForst1 and myself!
link.springer.com/book/10.10…
3
12
1,800
Digvijay Singh retweeted
It is great to see the #MicroED field growing and gaining momentum. I am aware of 5 new PIs that were hired this year. I am proud to say that 4 of those talented individuals were postdocs from our lab & they are now off to start their own groups. So proud of their achievements 😁
2
3
71
3,886
Digvijay Singh retweeted
Tiny gatekeepers of our nucleus decide what gets in and out. Follow our study on Yeast and Mammalian NPC baskets and unravel the molecular structure of these cellular bouncers! Don't miss the side-by-side comparison in the video. #IntegrativeModeling biorxiv.org/content/10.1101/…
Proud to share the result of an amazing collaboration with Javi Fernandez-Martinez, @RoutLab_RU , and @TheVillaLab ! biorxiv.org/content/10.1101/… Congrats to @dgvjayS @neeleshsoni03 @JoshHutchings94 @ig_ech and the team!
And check out the video by @neeleshsoni03 ! #NPC
2
10
47
10,702
Excited to share our manuscript on the in-cell structures and models of the nuclear basket (of nuclear pore complex; NPC) from both yeast and mammals!
+
Structural variations of NPCs across yeast, mammals, and protozoa.
Will do a tweetorial later!
biorxiv.org/content/10.1101/…
It cannot be done, they said.. It's too flexible, they said.. 😁
biorxiv.org/content/10.1101/… #NPC #teamtomo #integrativemodeling by @dgvjayS @neeleshsoni03 @JoshHutchings94 @ig_ech et al. Great collaboration w/ Qiang Guo, Brian Chait, Javi Fernandez-Mtnz, @RoutLab_RU @salilab_ucsf
6
14
127
13,858
Digvijay Singh retweeted
Proud to share the result of an amazing collaboration with Javi Fernandez-Martinez, @RoutLab_RU , and @TheVillaLab ! biorxiv.org/content/10.1101/… Congrats to @dgvjayS @neeleshsoni03 @JoshHutchings94 @ig_ech and the team!
And check out the video by @neeleshsoni03 ! #NPC
2
23
112
12,365
Digvijay Singh retweeted
Aissam Dam, 11, was the first person to get gene therapy in the U.S. for congenital deafness. The treatment was a success, introducing a child who had known nothing of sound to a new world.
“There’s no sound I don’t like,” he said. nyti.ms/47LmrPo
47
78
316
108,991
Digvijay Singh retweeted
Shocking Sight: Trophy Hunter Brittany L. killed this beautiful male leopard in his prime.😱😡
Let’s make this women famous 👇
Retweet and spread this far and wide.
4,159
8,033
17,010
40,416,959
Congrats to Barak et al. for this tour de force on modeling the nucleocytoplasmic transport! happy to make a small contribution to it!
New year, new manuscript! Our work on mapping transport between the nucleus and the cytoplasm is now on BioRxiv, and it's time to embark on a journey through the nuclear pore complex. Nuclear what? Bear with me!
biorxiv.org/content/10.1101/…
1
3
745