tries to make microscopes smarter · bioimage analysis, optogenetics, ml, 3d printing, open science · starting a lab, more info soon!
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Automated optogenetic control of hundreds of cells in parallel. Each cell is individually steered, collectively acting as a "tissue printer". Preprint & code out!
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Lucien Hinderling retweeted
300,000-500,000 USD vs 3,000-4,000 USD. A high-end AFM next to a DIY one built on a DVD pickup. Not a replacement, but nanoscale imaging for classrooms and small labs. Open access review in JJAP:
doi.org/10.35848/1347-4065/a…
#HardwareHacking #AFM
ALT Comparison table of a high-end commercial AFM (300,000 to 500,000 USD) and a compact DIY AFM built on a DVD optical pickup (3,000 to 4,000 USD), with photos of both
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Lucien Hinderling retweeted
Hope to see you online!
We are excited to invite you to the second webinar in our cell migration series. In this webinar, we focus on new models for studying cell migration with talks from Ankita Jha @Ankita_13Jha, Raphaël Clément & Katrina Velle @KatrinaVelle.
For more info⤵️: focalplane.biologists.com/20…
ALT FocalPlane features... emergent models and quantitative analysis of cell migration Thursday 17 September, 15:00-16:30 BST (UTC+1) Organised by Organised by Pablo J. Sáez and Valeria Venturini FocalPlane logo Picture of Ankita Jha (National Institutes of Health, USA) Decoding bleb driven signaling in cancer disease progression Picture of Raphaël Clément (L’Institut de Biologie du Développement de Marseille (IBDM), France) Navigating without neurons or muscles: Locomotion in Trichoplax Picture of Katrina Velle (University of Massachusetts Dartmouth, USA) Naegleria amoebae seek confinement and crawl persistently through narrow spaces #FocalPlaneFeatures focalplane.biologists.com Image of Naegleria.
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Lucien Hinderling retweeted
📣 new preprint multimodal atlas. Imaging + scRNA, 57M cells. 🧬🔬
Cells are complex dynamical systems — but most ways we measure them destroy them. We asked: how does live imaging compare to scRNA-seq, the field’s gold std?
The answer surprised us 🧵
biorxiv.org/content/10.64898…
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Lucien Hinderling retweeted
🔬The authors of this new paper demonstrate multimodal imaging of live cell co-cultures, including quantitative phase imaging (QPI) through differential phase contrast (DPC) imaging.
"Multimodal illumination for quantitative transmitted-light microscopy" doi.org/10.1111/jmi.70156
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Lucien Hinderling retweeted
animated version
August's Molecule of the Month is Histone Ubiquitination!!! Here is H2B K120 Ubiquitin. PDB: 8V25
Made in Blender with #MolecularNodes
Red is Ubiquitin
pdb101.rcsb.org/motm/320
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"In the late stages of Kimi K3 development, an early version of Kimi K3 handled the majority of the team's kernel optimization works."
Releasing the model weights and technical report of Kimi K3.
Kimi K3 is our most capable model: a 2.8T MoE model with native visual understanding and a 1M-token context window.
New model architecture: 2.5x the intelligence per unit of compute, not just more params.
Alongside Kimi K3, we're opening up more of the stack behind it — high-performance attention kernels, MoE communication library, and infrastructure for running agent environments at scale.
Model weights: huggingface.co/moonshotai/Ki…
Tech report: github.com/MoonshotAI/Kimi-K…
Tech blog: kimi.com/blog/kimi-k3
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Lucien Hinderling retweeted
I should also point out that there are other *excellent* steps in the "smart microscopy" + optogenetics direction, including recent efforts by the Pertz and Kapitein labs, that are reviewed here. Take a look!
degruyterbrill.com/document/…
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Lucien Hinderling retweeted
New Toettchlab paper alert! Harrison Oatman just published his beautiful work combining software design for "smart" microscopy+optogenetics and some really beautiful work understanding EGFR-driven collective cell migration.
cell.com/cell-systems/fullte…
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Lucien Hinderling retweeted
🎥 Don't miss this recording of the #VirtualPub with Lucien Hinderling @lhinderling
@PertzL on Smart Microscopy workflows - for automation of optogenetic targeting & beyond. Great talk 👏 🔽
piped.video/YpGmMzrFJ80
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Lucien Hinderling retweeted
JCS snapshot - Tracking coordinated cellular dynamics in time-lapse microscopy with ARCOS.px
Maciej Dobrzyński @macdobry presents the key findings from their recent JCS paper with Benjamin Grädel @benigraedel and colleagues @PertzL.
journals.biologists.com/jcs/…
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Won best thesis award with this video! 4 years of research and play in 3 minutes :')
piped.video/so0fPlK1-LM
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Lucien Hinderling retweeted
🥳🍾 Super excited to share one of the last papers from my PhD, out today in @Matter_CP. In this work, we introduce a very fast-relaxing (half time of ~seconds) *and* photopolymerizable hydrogel that allows for projection-based cell encapsulation in dynamic matrices. Link below!
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Last-minute advert for the Convpaint workshop at I2K by Roman Schwob! Interactive segmentation in @napari_imaging . Today 2:00 PM CET. Free registration:
i2kconference.org/workshops
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Lucien Hinderling retweeted
Fantastic paper by @JamesBeanBio on phage Bas63 - this has been one of the first phages I ever isolated just for fun as a PhD student (in 2012!). It's #2 on this plate of Rhine river water on an E.coli K-12 lawn....
How it started /// How it's going
Most recent paper out now at science advances. We investigated a BASEL phage belonging to the felixounavirus genus and discovered evolutionary relationships with distantly related phages. Collaborative work between @HiCryoEM and @mihnea_bostina
science.org/doi/10.1126/scia…
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microfabricated stamps, freshly printed :)
PAPER OUT ✨ "hack" your microscope to work as a 3D printer! micrometer sized features at cm scale. <5$ in material costs per chip. from idea to experiment within a day. first paper of my phd in PertzLab @PertzL to make it through peer review! doi.org/10.1039/D5LC00181A
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Lucien Hinderling retweeted
A real-time all-optical interface for dynamic perturbation of neural activity during behavior: sciencedirect.com/science/ar…
Real-time interface that integrates CaImAn for online calcium imaging analysis, and a custom hologram program for two-photon optogenetic stimulation.
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Lucien Hinderling retweeted
🚀 Excited to share scPortrait! Led by Sophia Mädler & Niklas Schmacke w/ the Mann lab — a new @scverse tool for standardized single-cell image data. Enables ML-ready extraction, >1B cell processing, cross-omics, & cancer macrophage insights.
🔗 biorxiv.org/content/10.1101/…
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Lucien Hinderling retweeted
In 2005, over 20 years ago I built an OPA and doubled it to play with all UV CARS, 20 years later, using left over parts and a laser I built a simple UV spectroscopy apparatus, if funding returns then I hope to return to deep UV and CARS... someday
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Lucien Hinderling retweeted
Just posted on bioRxiv: A novel model revealing how immune cells combine a ‘nuclear piston,’ uropod, and surroundings to power migration via a hydraulic cell engine. #CellBiophysics #ImmuneMigration #biorxiv_neursci biorxiv.org/content/10.1101/…
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