ALT Filamentation of C. albicans strains on solid agar. C. albicans cells from a YPD overnight culture were washed, and 105 cells of each strain were spotted on (A) chocolate agar, (B) Spider agar, or (C) YPD agar. Plates were incubated at 37°C for 5 days. Each medium induced wrinkling in the center of the colonies and extensive hyphae formation by the C. albicans wild-type SC5314. The hma1Δ/Δ mutant strains were severely impaired in formation of the hyphal fringe, but central wrinkles remained. The scale bar represents 0.5 cm.

ALT (A) Number of plates with no growth or growth (human truth at 48 h) among all specimens used in the study. (B and C) Overall, sensitivity increased over time while specificity remained constant. Error bars represent 95% CI. (D) Confusion matrix, compared at all timepoints, and the prediction of growth of the AI (x axis) against the human (y axis). The number of FP and FN prediction is respectively shown in orange and red plots. At 48 h, 2,451 plates showed a concordant growth prediction, while 5 were not predicted by the AI and 111 were FP calls. A reading at 20 h by the AI shows 2,334 perfect matches between the human and the AI, with 27 FN and 93 FP calls. Out of the 27 FN, 5 are later predicted at 22 h, 7 at 24 h, 5 at 36 h, and 1 at 48 h (red dashed lines). Conversely, out of the 93 FP calls, 13 were stated as growth by the human at 22 h, 5 at 24 h, 8 at 36 h, and 2 at 48 h (orange dashed lines).

ALT a person sitting at a desk with a pile of agar plates and next to a full biohazard bin